Florida State University

Dr. Timothy Megraw is currently accepting new students.

Tmegraw c87e445cc95507b414e643f969ed672ec1f059097b34aaac4c93005a9a5e3bb8

Dr. Timothy Megraw

Associate Professor of Biomedical Sciences & Neuroscience

Faculty
Biomedical Sciences
MSR 3300
645-9271

tmegraw@neuro.fsu.edu

Interest
The Megraw lab investigates the functions of centrosomes and cilia in cell division, development and disease using Drosophila, mouse, and human cell culture models. This includes asymmetric division of stem cells, the regulation of centrosomal and other microtubule-organizing centers (MTOCs), metabolic disorders due to loss of centrosome proteins, and regulation of primary cilium assembly and function. Centrosome-based disorders that impact stem cell function and brain development are a major focus of our research.
Current Research
The centrosome is the major microtubule-organizing center (MTOC) in animal cells and impacts many disease-related functions. We focus on the links between centrosome functions and inherited forms of microcephaly that arise from syndromes such as MCPH or SCKL. Our investigations show cell division-related defects in stem cells arising from impaired centrosome replication, but there are also specific metabolic impairments in cells mutant for centrosome protein genes. We suspect that Zika virus perturbs the same centrosome-related pathways in human neural stem cells, causing microcephaly in infected mothers during their pregnancy. We are investigating the links between Zika virus infection and centrosome impairment, to connect the inherited and viral-induced forms of microcephaly.
Recent Publications
Guarderas F, Leavell Y, Sengupta T, Zhukova M, Megraw TL, Assessment of Chicken-Egg Membrane as a Dressing for Wound Healing, Adv Skin Wound Care, 2016 PubMed
Jodeiri Farshbaf M, Ghaedi K, Megraw TL, Curtiss J, Shirani Faradonbeh M, Vaziri P, Nasr-Esfahani MH, Does PGC1alpha/FNDC5/BDNF Elicit the Beneficial Effects of Exercise on Neurodegenerative Disorders?, Neuromolecular Med, 2016 PubMed
Bingol K, Li DW, Bruschweiler-Li L, Cabrera OA, Megraw T, Zhang F, Brüschweiler R, Unified and isomer-specific NMR metabolomics database for the accurate analysis of (13)C-(1)H HSQC spectra, ACS Chem Biol, 2015 PubMed
Man X, Megraw TL, Lim YP, Cep68 can be regulated by Nek2 and SCF complex, Eur J Cell Biol, 2015 PubMed
Tözser J, Earwood R, Kato A, Brown J, Tanaka K, Didier R, Megraw TL, Blum M, Kato Y, TGF-beta Signaling Regulates the Differentiation of Motile Cilia, Cell Rep, 2015 PubMed
Chen JV, Kao LR, Jana SC, Sivan-Loukianova E, Mendonça S, Cabrera OA, Singh P, Cabernard C, Eberl DF, Bettencourt-Dias M, Megraw TL, Rootletin organizes the ciliary rootlet to achieve neuron sensory function in Drosophila, J Cell Biol, 2015 PubMed
Chen JV, Megraw TL, Spermitin: a novel mitochondrial protein in Drosophila spermatids, PLoS One, 2014 PubMed
Chen JV, Megraw TL, Mother centrioles do a cartwheel to produce just one daughter, Dev Cell, 2014 PubMed
Galletta BJ, Guillen RX, Fagerstrom CJ, Brownlee CW, Lerit DA, Megraw TL, Rogers GC, Rusan NM, Drosophila pericentrin requires interaction with calmodulin for its function at centrosomes and neuronal basal bodies but not at sperm basal bodies, Mol Biol Cell, 2014 PubMed
Riparbelli MG, Cabrera OA, Callaini G, Megraw TL, Unique properties of Drosophila spermatocyte primary cilia, Biol Open, 2013 PubMed
Chen JV, Megraw TL, Cenexin1 and Odf2: splice variants with diverged cilium functions, Cell Cycle, 2013 PubMed
Kao LR, Megraw TL, Cytokinesis: RhoGEFs control a developmental cleavage switch, Curr Biol, 2012 PubMed
Riparbelli MG, Callaini G, Megraw TL, Assembly and persistence of primary cilia in dividing Drosophila spermatocytes, Dev Cell, 2012 PubMed
Megraw TL, Sharkey JT, Nowakowski RS, Cdk5rap2 exposes the centrosomal root of microcephaly syndromes, Trends Cell Biol, 2011 PubMed
Megraw TL, PP2A targets SAS-5 in centriole assembly, Dev Cell, 2011 PubMed
Gopalakrishnan J, Mennella V, Blachon S, Zhai B, Smith AH, Megraw TL, Nicastro D, Gygi SP, Agard DA, Avidor-Reiss T, Sas-4 provides a scaffold for cytoplasmic complexes and tethers them in a centrosome, Nat Commun, 2011 PubMed
Barrera JA, Kao LR, Hammer RE, Seemann J, Fuchs JL, Megraw TL, CDK5RAP2 regulates centriole engagement and cohesion in mice, Dev Cell, 2010 PubMed
Kao LR, Megraw TL, Centrocortin cooperates with centrosomin to organize Drosophila embryonic cleavage furrows, Curr Biol, 2009 PubMed
Mottier-Pavie V, Megraw TL, Drosophila bld10 is a centriolar protein that regulates centriole, basal body, and motile cilium assembly, Mol Biol Cell, 2009 PubMed
Kotadia S, Kao LR, Comerford SA, Jones RT, Hammer RE, Megraw TL, PP2A-dependent disruption of centrosome replication and cytoskeleton organization in Drosophila by SV40 small tumor antigen, Oncogene, 2008 PubMed
Zhang J, Megraw TL, Proper recruitment of gamma-tubulin and D-TACC/Msps to embryonic Drosophila centrosomes requires Centrosomin Motif 1, Mol Biol Cell, 2007 PubMed
Kao LR, Megraw TL, RNAi in cultured Drosophila cells, Methods Mol Biol, 2004 PubMed
Megraw TL, Kilaru S, Turner FR, Kaufman TC, The centrosome is a dynamic structure that ejects PCM flares, J Cell Sci, 2002 PubMed
Megraw TL, Kao LR, Kaufman TC, Zygotic development without functional mitotic centrosomes, Curr Biol, 2001 PubMed
Megraw TL, Kaufman TC, The centrosome in Drosophila oocyte development, Curr Top Dev Biol, 2000 PubMed
Megraw TL, Li K, Kao LR, Kaufman TC, The centrosomin protein is required for centrosome assembly and function during cleavage in Drosophila, Development, 1999 PubMed
Li K, Xu EY, Cecil JK, Turner FR, Megraw TL, Kaufman TC, Drosophila centrosomin protein is required for male meiosis and assembly of the flagellar axoneme, J Cell Biol, 1998 PubMed
Cho JH, Ha SJ, Kao LR, Megraw TL, Chae CB, A novel DNA-binding protein bound to the mitochondrial inner membrane restores the null mutation of mitochondrial histone Abf2p in Saccharomyces cerevisiae, Mol Cell Biol, 1998 PubMed
Megraw T, Kaufman TC, Kovalick GE, Sequence and expression of Drosophila Antigen 5-related 2, a new member of the CAP gene family, Gene, 1998 PubMed
Kao LR, Megraw TL, Chae CB, SHM1: a multicopy suppressor of a temperature-sensitive null mutation in the HMG1-like abf2 gene, Yeast, 1996 PubMed
Megraw TL, Kao LR, Chae CB, The mitochondrial histone HM: an evolutionary link between bacterial HU and nuclear HMG1 proteins, Biochimie, 1994 PubMed
Kao LR, Megraw TL, Chae CB, Essential role of the HMG domain in the function of yeast mitochondrial histone HM: functional complementation of HM by the nuclear nonhistone protein NHP6A, Proc Natl Acad Sci U S A, 1993 PubMed
Megraw TL, Chae CB, Functional complementarity between the HMG1-like yeast mitochondrial histone HM and the bacterial histone-like protein HU, J Biol Chem, 1993 PubMed

All Publications PubMed

Lab Website

Lab Members

Jieyan Chen, Postdoctoral Associate

Ling-Rong Kao, Affiliate

Return to List